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KCAS Bioanalytical and Biomarker Services
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Revvity
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Diaclone
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Image Search Results
Journal: Journal of Applied Oral Science
Article Title: Cytidine-phosphate-guanosine oligodeoxynucleotides in combination with CD40 ligand decrease periodontal inflammation and alveolar bone loss in a TLR9-independent manner
doi: 10.1590/1678-7757-2017-0451
Figure Lengend Snippet: CpG increased IL-10 mRNA expression and protein secretion in spleen B cells from WT and TLR9 KO mice. Purified spleen B cells separated from WT mice and TLR9 KO mice were cultured with CD40L (0.1 μg/ml), CpG (1 μM) +CD40L (0.1 μg/ml) for 48 h. The levels of IL-10 mRNA expression (A) and protein secretion (C) were detected by qRT-PCR and ELISA, respectively (mean±SD, n=4 WT mice or TLR9 KO mice per group, *p<0.05, **p<0.01). Purified spleen B cells separated from WT mice and TLR9 KO mice were cultured with CpG-Low (0.1 μM), CpG-Med (1 μM) and CpG-High (10 μM) for 48 hours. The levels of IL-10 mRNA expression (B) and protein secretion (D) from cultured B cells were detected by qRT-PCR and ELISA, respectively (mean±SD, n=4 WT mice or TLR9 KO mice per group, *p<0.05, **p<0.01)
Article Snippet: We used Mouse IL-10 enzyme-linked
Techniques: Expressing, Purification, Cell Culture, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay
Journal: Disease Markers
Article Title: Identification of Gingival Crevicular Fluid Sampling, Analytical Methods, and Oral Biomarkers for the Diagnosis and Monitoring of Periodontal Diseases: A Systematic Review
doi: 10.1155/2016/1804727
Figure Lengend Snippet: Comparison of the mean of IL-1 β concentration between different studies using the same analytical techniques.
Article Snippet: ELISA kit , Standard ELISA kit (Bender Med Systems, Vienna, Austria) ,
Techniques: Comparison, Concentration Assay, Enzyme-linked Immunosorbent Assay